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KMID : 0043320050280111302
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Archives of Pharmacal Research
2005 Volume.28 No. 11 p.1302 ~ p.1310
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Glucosylated Polyethylenimine as a Tumor-Targeting Gene Carrier
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Park In-Kyu
Cook Seung-Eun
Kim You-Kyoung
Kim Hyun-Woo
Cho Myung-Haing
Jeong Hwan-Jeong
Kim Eun-Mi
Nah Jae-Woon
Bom Hee-Seung
Cho Chong-Su
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Abstract
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Glucosylated polyethylenimine (GPEI) was synthesized as a tumor-targeting gene carrier through facilitative glucose metabolism by tumor glucose transporter. Particle sizes of GPEI/DNA complex increased in proportion to glucose content of GPEI, whereas surface charge of the complex was not dependent on glucosylation, partially due to inefficient shielding of the short hydrophilic group introduced. GPEI with higher glucosylation (36 mol-) had no cytotoxic effect on cells even at polymer concentrations higher than 200 g/mL. Compared to unglucosylated PEl. glucosylation induced less than one-order decrease of transfection efficiency. Transfection of GPEI/DNA complex into tumor cells possibly occurred through specific interaction between glucose-related cell receptors and glucose moiety of GPEI. Gamma imaging technique revealed GPEI/DNA complex was distributed in liver. spleen. and tumors.
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KEYWORD
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Tumor-targeting, Glucose transporter, Glucosylation, Polyethylenimine
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